Equipment-Specific Terms of Use for the Laboratory for Electron Microscopy (LEM)

of the Department of Biology’s Imaging Center

Date: 28th July 2017

§ 1 Scope of the Terms of Use

These Terms of Use govern the use of services by LEM in both user and service operations. The Terms of Use are set out in this appendix which is incorporated into the General Terms of Use of the Department of Biology’s Imaging Center.

§ 2 Contacts

  1. The Head of LEM, Dr. Rabea Schlüter (rabea.schlueter@uni-greifswald.de), is the main contact for technical and scientific queries, information on the services available at LEM and advice to users and interested parties.
  2. Other contacts for technical queries are the delegated contacts for the equipment as listed on the Imaging Center’s website (https://imaging.uni-greifswald.de).

§ 3 LEM’s Scope of Services

  1. Equipment
    The following electron microscopes are available at LEM:
    • Scanning Electron Microscope
      • EVO LS10 (Carl Zeiss Microscopy GmbH)
    • Transmission Electron Microscope
      • LEO 906 (Carl Zeiss Microscopy GmbH)
    A detailed description of the microscopes is available on the Imaging Center’s website (https://imaging.uni-greifswald.de).

  2. Techniques for Sample Preparation
    The methods used to prepare samples are described on the Imaging Center’s website (https://imaging.uni-greifswald.de).

  3. Other Services On Offer
    LEM’s services include scientific advice to users by the Head of LEM with regard to the investigation at hand and the experiments required, how to prepare the samples for the electron microscope, microscopy and photographic documentation of the samples. The Scanning Electron Microscope is additionally equipped with an EDX system for energy dispersive x-ray spectroscopy enabling the elemental analysis of samples.

§ 4 Preparation of the Samples

  1. Only LEM staff members are allowed to prepare samples using the facilities of LEM.
  2. Users and external customers must ensure that the biological material used in the trials as well as samples which are handed in for microscopy or used in user operations are in an inactive form. They have to make sure that the samples themselves or the handling of them will pose no risk to humans.
  3. If, due to high demand, not all service requests can be met, the Head of LEM will decide on how capacity is allocated and in which chronological order individual service requests are dealt with. Priority will be given to the different user groups as per § 4 of the General Terms of Use.
  4. Samples from external customers are generally processed in the order in which they are received while also taking into account the best possible processing efficiency.

§ 5 General Use of the Equipment

  1. The equipment is usually operated by LEM staff members (service operation).
  2. In longer projects with a large amount of samples or with samples that require extensive imaging, it is also possible to allow users to operate the electron microscope themselves (user operation as per § 4.3 of the General Terms of Use).

§ 6 Admission to Equipment and Rules of Access for Operators

  1. Operators may not use the equipment specified under § 3 until they have filled in the questionnaire (§ 5.2 of the General Terms of Use) for that particular device (one questionnaire per device) and until they have been given a briefing by the Head of LEM or person who is in charge of the device. The briefing takes place over the course of several days. The briefing is only valid for the person to whom it has been given and is combined with a safety briefing. Only after this are operators allowed to use the electron microscopes on their own.
  2. Operators have to use the equipment at least once in every quarter. If the equipment was last used by the operator more than six months previously, the samples will then be examined with the microscope by the person responsible for the equipment and charged according to the fee table. If the microscope was last used by the operator more than six months ago and it is foreseeable that s/he will have to examine a high number of samples again, there will be a new briefing (including safety briefing) that will this time be charged.
  3. Appointments for the operators will be given out after consultation with the Head of LEM.
  4. All bookings are binding and will be charged. There are exceptions, however, for example if you cancel the appointment 24 hours in advance or if maintenance work is being carried out on the equipment or it is being repaired during the time that was allocated to you for use or if you were unable to use the equipment for reasons for which the LEM is responsible.
  5. If the equipment cannot be used due to a defect or because it is being serviced, LEM is entitled to cancel the appointment. Operators will be informed of this via email.

§ 7 Usage of Equipment by Operators

  1. Only authorised operators may use the equipment listed in § 3. Operators are strictly forbidden to grant access to the equipment to any third parties.
  2. Operators may only use the equipment during the allocated time slot. If the operator needs to use the equipment for a longer period, this can be arranged with the Head of LEM unless the equipment is already booked out for that time.
  3. The data generated by operators may only be copied from the computers connected to the equipment by LEM staff members using USB sticks supplied by them!
  4. At the end of use, the operator must fill in the logbook and sign it.

§ 8 Storing Samples

  1. The samples that have been prepared or treated for the Scanning Electron Microscope will be stored in the exsiccator and will normally be disposed after a storage period of five years.
  2. The grids prepared for the transmission electron microscope will be stored in the exsiccator and will normally be disposed after a period of five years.

Appendix

LEM’s currently valid fee table

 

The translation of these regulations is to be seen as a reference and to be used for the understanding of the corresponding German document. Only the German version of the original document is legally binding.

Wherever used herein, gender-specific terms should be understood as referring to both genders