Equipment-Specific Terms of Use for Fluorescence Microscopes

of the Department of Biology’s Imaging Center

at the Institute of Microbiology

Date: 28th July 2017


§ 1 Scope of the Terms of Use

  1. These Terms of Use govern the handling of fluorescence microscopes at the Institute of Microbiology in user operations. The Terms of Use are set out in this appendix which is incorporated into the General Terms of Use of the Department of Biology’s Imaging Center.
  2. Use in service operations is subject to the regulations set out in the General Terms of Use.

§ 2 Contacts

  1. General Contact:  Dr. Rabea Schlüter (
  2. Prof. Dr. Katharina Riedel ( is a member of the Imaging Center’s Steering Committee and the contact for all matters concerning fluorescence microscopes at the Institute of Microbiology.
  3. Besides Prof. Riedel, the main contact for all technical and scientific queries to do with the services offered and also responsible for advice to users or interested parties: Dr. Jan Pané-Farré (
  4. Further contacts for technical enquiries are the delegated contacts in charge of the equipment as specified on the Imaging Center’s website (

§ 3 Equipment

  1. The following fluorescence microscopes are available at the Institute of Microbiology:
    • Fluorescence Microscope
      • Zeiss Axio Imager M2
    • Epifluorescence Microscope
      • Deltavision DeltavisionRT Image restoration workstation
    • Confocal Laser Scanning Microscope
      • Zeiss LSM 510 META
  2. A detailed description of the microscopes is available on the Imaging Center’s website (

§ 4 Admission to Equipment and Rules of Access for Operators

  1. Operators may not use the equipment specified under § 3 until they have filled in the questionnaire (§ 5.2 of the General Terms of Use) for that particular device (one questionnaire per device) and until they have been briefed by Dr. Pané-Farré or another person in charge of the equipment. The briefing is only valid for the person to whom it has been given and needs to be accompanied by a laser safety briefing (if the equipment mentioned in § 3.1 is fitted with lasers) and a safety briefing which may only be carried out by a laser safety officer.  After briefing, operators will be set up in the booking system and granted access to the rooms where the equipment is kept. Usage is limited to a period of one year. After that, the operator must receive a new laser safety briefing (if the equipment mentioned in § 3.1 is fitted with lasers) and will then be re-activated in the booking system. Operators who haven’t used the equipment for over six months will not continue to be authorised unless they are given another laser safety briefing (if the equipment mentioned in § 3.1 is fitted with lasers) and are again shown how to use the equipment.
  2. Once the equipment briefing and general safety and laser safety briefings are complete, access to the rooms where the equipment is situated will be activated on the operator’s staff ID card or a guest ID card.
  3. The equipment may only be used and access to the rooms where it is situated only granted if a briefing according to § 14 of the Biostoffverordnung (Biological Agents Ordinance, BioStoffV) and § 12 of the Gentechnik-Sicherheitsverordnung (Genetic Technology Safety Ordinance , GenTSV) has taken place and if an occupational health check according to § 6 of the Verordnung zur arbeitsmedizinischen Vorsorge (Ordinance on Occupational Health Care, ArbMedVV) has been given. All these must be confirmed by personal signature on the questionnaire.
  4. A guest ID card is available from Dirk Albrecht ( The operator has to sign for receipt of the card. Once the task has been completed, the ID card has to be returned in person to Dr. Albrecht.

§ 5 Equipment Booking Procedure for Operators

  1. Microscopes can be booked via the calendar option of the Groupware webmail system at the University of Greifswald. Operators must ensure that their passwords are protected against misuse by any third parties.
  2. Only authorised operators may make bookings. Operators are strictly forbidden to book equipment for third parties who have not been briefed or for any other unauthorised persons. Non-compliance can result in immediate loss of their own permit.
  3. Bookings that cover a period of more than two consecutive days must first be cleared with Dr. Pané-Farré.
  4. The equipment can only be used if it has been booked electronically in advance.
  5. All bookings are binding and will be charged.  There are exceptions, however, for example if you cancel the booking 24 hours in advance or if maintenance work is being carried out on the equipment or it is being repaired during the time that was allocated to you for use or if you were unable to use the equipment for other reasons for which the Institute of Microbiology is responsible.
  6. All operators from the Institute of Microbiology and the Interfaculty Institute for Genetics and Functional Genomics who have access to the Institute of Microbiology’s alarm system can access the equipment at any time day or night.
  7. For all other operators, the equipment is available Monday to Friday (except public holidays) from 7.30 a.m. to 6.30 p.m.
  8. It is not permissible to use the equipment without first booking it via the Groupware webmail system.
  9. If the equipment cannot be used due to a defect or because it is being serviced, then either no booking will be possible or it will be cancelled by the person responsible for the equipment (§ 2). Operators will be informed of this via email.

§ 6 Equipment Use by Operators

  1. Only authorised operators may use the equipment listed in § 3.
  2. The equipment can only be used during the booked time. If the experiments require a longer period of use, the booking period can be directly extended unless the time slot has already been allocated to somebody else.
  3. After use, the work area must be left clean and tidy (i.e. the lenses and the work surface must be cleaned, used material and any leftover glass must be discarded). The work surface in front of the microscope must be disinfected with the surface disinfectant provided. Infectious samples must be disposed of by the work groups as per the instructions of the operator’s laboratory.
  4. The last operator of the day must switch off the system. Every operator has to check in the Groupware webmail calendar if there are any other operators after him/her. If the next operator is not due for any period of over an hour, the lasers and the UV lamp must be switched off.
  5. At the end of use, the operator must fill in the logbook and sign it.


Currently valid fee table for the use of fluorescent microscopes at the Institute of Microbiology



The translation of these regulations is to be seen as a reference and to be used for the understanding of the corresponding German document. Only the German version of the original document is legally binding.

Wherever used herein, gender-specific terms should be understood as referring to both genders.